This newly introduced screening system can be regarded as an effective and reliable in-vitro method for screening a tremendous number of hair growth candidates in a short period of time. In this method, primary dermal papilla cells and neonatal keratinocytes are co-cultured on a micro-patterned 3D spheroid 96-well culture plate with capacity of 60 spheroids/well resulting in the formation of numerous uniform-sized 3D compact spheroids. The efficacy of the test drugs on keratinocyte pillars growth is assessed using fluorescence immunostaining techniques, and analysis the image data by a high-content screening (HCS) instrument. This approach is verified by testing the hair-stimulating drugs such as Minoxidil which has showed significant elongation of the keratinocyte pillars, making it a promising alternative to animal tests.

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